Description
Intended Uses: This sandwich kit is for the accurate quantitative detection of Rat 5-hydroxytryptamine Receptor 1A (also known as HTR1A) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat HTR1A antibody. HTR1A present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat HTR1A Antibody is added and binds to HTR1A in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated HTR1A antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat HTR1A. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm